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KMID : 0385520130260040262
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Analytical Science & Technology
2013 Volume.26 No. 4 p.262 ~ p.267
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Separation and growth monitoring of oyster mushroom spores using gravitational field-flow fractionation (GrFFF)
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Kim Sun-Tae
Ji Eun-Young
Lee Seung-Ho
Lee Jae-Chan
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Abstract
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Gravitational field-flow fractionation (GrFFF) is a separation technique that utilizes earth‘s gravity as the external field. GrFFF is a convenient tool for the size and/or density-based separation of micron-sized particles of various origins. In this study, GrFFF was employed for size-based separation of oyster mushroom spores. Oyster mushroom spores have smooth surface and are of cylindrical to narrow kidney-shapes with 5 to 12 im in longer dimension and 3 to 4 im in shorter dimension, as was confirmed by optical microscope (OM). GrFFF conditions were optimized for separation and characterization of spores by varying the channel flow rate from 0.5 to 1 mL/ min. During the GrFFF elution of the spores, 3 fractions were collected to confirm the growth of oyster mushroom spore. The collected fractions were incubated for 30 days in water to examine the influence of the size on the growth of the spores. Results suggested that the oyster mushroom spores collected at the middle part of the GrFFF fractogram grew faster than those collected at the beginning or at the end of the fractogram.
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KEYWORD
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gravitational field-flow fractionation (GrFFF), oyster mushroom spore, Size distribution,
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